Elucidating protein structure

Crystallography starts from a crystal and to get crystals the protein needs to be crystallized.People often say: Crystallization is an art and not a science.

may also be used for obtaining three-dimensional structural information of protein structures.Confirmation of protein identity through structural characterisation facilitates the understanding of the molecule’s molecular structure, from the primary amino acid sequence to the high order structure.Researchers want to be able to isolate structural effects of a protein during drug binding or interaction with substrates/inhibitors.These interests have led to the development of methodology that relies heavily on synthetic and analytical chemistry to identify protein-small molecule and protein-metabolite interactions.